The OPU procedure for mares is performed at our clinic. Prior to the procedure, the mare is sedated and then her follicles are punctured, allowing several eggs to be collected.
Immediately after collection, the eggs are studied in the laboratory to determine which are qualitatively suitable for use in the ICSI procedure. On average, 60% of eggs obtained are often usable.
The usable portion of the eggs is then transported to Italy, where the exclusive frozen semen is stored.
Once in Italy, a special fluid is added to the eggs to ensure that they mature and subsequently can be injected with the selected sperm cells.
Next, a small portion of a frozen semen straw is cut and thawed, after which the semen quality is tested. The strongest sperm cells are used to fertilize the eggs.
After the eggs are fertilized, they are incubated for seven days. During this period, we check that an embryo is developing.
Once an embryo has formed, it is frozen and sent to Utrecht, where it is stored until the embryo is transferred into the recipient mare.
When the recipient mare ovulates in the spring, the embryo is thawed and transferred into that mare in which it further develops.